2023-07-20 

3D trajectory data for 
"Physiological adaptation in flagellar architecture improves Vibrio alginolyticus chemotaxis in complex environments"
in press at PNAS
by
Marianne Grognot, Jong Woo Nam, Lauren E. Elson & Katja M. Taute*

*taute@rowland.harvard.edu

An earlier version of the manuscript is available at 
https://www.biorxiv.org/content/10.1101/2023.02.06.526967v1


The .mat files provide the trajectory datasets 0 to 7 as defined in Tables S1 and S2 in the Supplementary Information, in a Matlab structure format. 
Trajectory position data are ADMM filtered as described in the Methods section of the manuscript.

Each file contains multiple Matlab structures starting with "V_P" (for the Pof-only strain) or "V_PL" (for the WT strain), followed by dataset name (e.g., "1a") as defined in the SI Tables.

Each structure V contains the following fields:
* V.Speeds is a cell array where each cell corresponds to one trajectory. The trajectory is described by an array with the following columns:
	- column 1: frame number
	- column 2-4 : x, y and z positions (in micrometers)
	- column 6-8 : instantaneous speed in x, y and z (micrometers per second)
	- column 9 : absolute value of instantaneous speed (micrometers per second)
	- column 10 : local rate of angular change, as defined in Methods (degrees/frame)
* V.Parameters is a simplified structure with descriptive parameters, containing: 
	V.Parameters.fps : frame rate of the recording (frames per second)
	V.Parameters.medium : the medium filling the gradient channel of the chemotaxis chamber (Dataset 1-6) or the motility chamber (Dataset 7).
	(optional) V.Parameters.growth : indicates the growth medium used, if the strains were grown in another medium than MB + 7% PVP.
